In 1598 the Padovan nobleman Francesco Gasparini, together with his wife Giuliana and daughter Virginia, founded by will a place "in the guise of a monastery" for the reception of poor maidens, which was called Zitelle Gasparine, to distinguish it from that of the Zitelle del Vescovo, an institute of similar purpose set up by the Bishop of Padova Marco Cornaro in the same years (Portenari 1623, p. 484).
The building was built in the district of the Pozzo della Vacca (which would later become the district of the Zitelle, after the name of the institution), in the property of Gasparini and a church dedicated to the Holy Trinity was annexed to it, on the façade of which there is a plaque commemorating the founder.
The conservatory remained in conformity with the first institution until 1825, when it was transformed into a boarding school to educate noble girls. At that time its rooms counted three large dormitories, three rooms used as classrooms, rooms for the teachers and administrators of the institute and a refectory, and 30 girls were accommodated there.